Abstract
D-type cyclins (CYCD) respond to various signals and control the G1/S progression. We have previously demonstrated that one of CYCDs, CYCD4;1 might be involved in not only G1/S progression but also G2/M progression, suggesting that CYCD4;1 has unique function among the CYCD families. In the Aabidopsis genome, CYCD4;2 that shares about 55 % similarity to CYCD4;1 at the amino acid level is also present.
To elucidate the function of CYCD4 in plant development, we first studied the spatial expression patterns of both CYCD4;1 and CYCD4;2. The results showed that both genes were expressed in particular tissues, such as vasculature and anthers. Next, we isolated T-DNA tagged lines of both genes and found that early leaf senescence was seen in the line of CYCD4;2 although stem and flower development seemed to be normal.
