Abstract
Over 10,000 non-redundant Arabidopsis full length cDNA (fl-cDNA) clones were mixed to make a normalized fl-cDNA over-expression T-DNA library. Nearly all the cDNA was cloned in the sense orientation relative to the expression promoter. Agrobacterium was transformed with the normalized T-DNA library to give the corresponding Agrobacterium library. A. thaliana col. WT plants were dipped with the Agrobacteria library. 800 transformed plants were grown in green house to isolate 80 plants, which showed obvious phenotypes such as morphological abnormality, greening and pigmentation. PCR was performed from 40 of these lines using vector specific primers. The PCR fragments were sequenced to find out that all these 40 fl-cDNA inserted newly in the genome was not redundant. We designated this activation tagging-like system as Fox Hunting System (full-length cDNA over-expressor gene hunting system) to develop it as a novel technology for the functional genomics in plant science.
