Database of RIKEN Arabidopsis full-length cDNAs and Ds transposon insertion lines

Abstract

  Full-length cDNA is essential for identification of gene function in post sequencing era. Until now, we have collected RIKEN Arabidopsis full-length(RAFL) cDNAs and Ds transposon insertion lines. We have determined the full-length cDNA sequences of 12,189 clones in collaboration with the Arabidopsis SSP group of the USA as of December 2002. We also constructed promoter database by comparing the 5'-end sequence of the full-length cDNAs with the genomic sequence data. We are planning to construct the comprehensive basic database of expression profiles in Arabidopsis by combining microarray results with the promoter database. Furthermore, we constructed the database of 9,236 Ds transposon insertion lines and are applying it to functional analysis and phenome analysis.
  These data have been offered from the website (http://pfgweb.gsc.riken.go.jp/) of RIKEN GSC. In this meeting, we report our RIKEN Arabidopsis database.