Abstract
The nuclear genome of tobacco cultivar Nicotiana tabacum consists of two-type sub-genome (S-genome and T-genome), and these are derived from ancestors of N. sylvestris (S-genome donor) and N.tomentosiformis (T-genome donor). On the other hand, old data suggested that the progenitor N. sylvestris is chloroplast genome donor. To compare the chloroplast genomes in Nicotiana speices, DNA was prepared from isolated intact chloroplasts from N. sylvestris and N. tomentosiformis green leaves and determined the complete nucleotide sequences of the each chloroplast genome using shotgun sequencing method. Comparison of Nicotiana chloroplast genomes confirms that the chloroplast genome of N. tabacum was certainly derived from that of N. sylvestris.
