Abstract
In rice fzp mutant, spikelet meristems are transformed to branch shoots. Cloning of the FZP gene revealed that it is a member of ERF family which functions as a transcription factor. In situ hybridization analysis showed that FZP mRNA was expressed between rudimentary glumes and the spikelet meristem in young spikelets.
We asked if the FZP functions in a same way as ERF proteins that act as GCC box-mediated transcription factors. The FZP and a reporter gene containing the GCC box in promoter were introduced into leaves using particle gun. Expression of the reporter gene was increased by FZP. Next, FZP fused with GAL4 and a reporter gene containing GAL4 box in promoter were introduced into leaves. The rise of the reporter gene by FZP and GAL4 fusion protein was observed. These results demonstrated that the FZP acts as a GCC box-mediated transcription activator.
