Abstract
Almost all genes express in circadian manner in cyanobacteria. We developed dual-reporter system for continues monitoring two different promoters in living cell of single strain. We selected two luciferases of railroad-worms with different emission spectra: PvGR-luc emits green and PhRE-luc emits red bioluminescence.
We constructed the reporter strains in which PhRE-luc fused with promoter of kaiBC, cyanobacterial clock gene and PvGR-luc fused with promoter of trc, E. coli gene. And the bioluminescence was monitored with interference filters. Two bioluminescences were separated effectively. Both of luciferases construct exhibit the circadian rhythms as well as Vibrio luciferase, which has been mainly used in circadian rhythm study of cyanobacteria. It is demonstrated that luciferases of railroad worms are useful for monitoring the circadian gene expression in a single cyanobacterial cells and such dual-reporter system enable us to study the activities of individual promoters exactly in living cell.
