Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 44
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Isolation and Analysis of Ozone-Sensitive Arabidopsis Mutants
*Mitsuko AonoMachi KannaTomoko KawashimaAkihiro KuboMasanori TamaokiNobuyoshi NakajimaHikaru Saji
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Pages 499

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Abstract
Ozone-sensitive Arabidopsis mutants are used to clarify the mechanisms of plant damage caused by ozone. Nine mutant lines were selected from mutagenized Col gl1 and Ws-2 by visible foliar injury during ozone exposure as an indicator. These mutant lines showed the similar sensitivity to UV-B to the control, however, they revealed various sensitivities to other environmental stressors, such as low temperature, high-intensity light, a herbicide paraquat, or SO2. This implies that different causes of ozone sensitivity exist among these mutants. Rough-mapping using molecular makers showed the 4 lines of Col-background mutants have the causative locus on different chromosomes. Ethylene is known to be involved in damage caused by ozone. Two of Col-lines exhibited higher ethylene emission than the control during ozone exposure. High ethylene emission may increase the damage in these lines. No difference in ascorbate contents among the mutants and the control.
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© 2003 by The Japanese Society of Plant Physiologists
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