Abstract
We isolated a salt-stress inducible gene, AcPMP3, from salt-stressed Aneurolepidium chinense leaves cDNA library. Aneurolepidium chinense is a wild perennial plant, growing naturally in the semiarid region of Mongolia. It is worth while studying on adaptation mechanisms of salt-tolerance in the plant, because Aneurolepidium chinense can grow under high saliniry condition (500 mM NaCl).
The deduced ORF of AcPMP3 is found to encode a highly hydrophobic protein (54 amino acids), which is predicted to contain two membrane-spanning segments.
Northern analysis showed that AcPMP3 gene is induced not only under salt stress but also other stresses such as low temperature and dehydration.
The AcPMP3 mRNA was detected at root epidermis and tip. This gene seems to be very important for salt-stress tolerance, because these organs are directly exposed to the high-salt environment.
The yeast cells harboring AcPMP3-GFP fusion protein showed that this protein is localized at cell membrane.
