Abstract
To understand the mechanism of cell-to-cell movement of regulatory protein, we are studying Myb protein, CPC, which positively regulates root hair formation in Arabidopsis.
We constructed the transgenic Arabidopsis harboring CPC:GFP fusion gene driven by CPC promoter. This line showed GFP fluorescence in the nuclei of both hair cell and hairless cell of root epidermis while mRNA is expressed only in hairless cell. This result indicated that CPC protein could move from the hairless cell to hair cell.
We are making several constructs in which truncated CPC was fused to GFP, and examining which region of CPC is responsible for cell-to-cell movement. To investigate the direction of CPC movement, CPC:GFP fuion protein was expressed in the enddermis or hair cell by SCR or MYC2 promoter, respectively. The results showed that GFP fluorescence was observed in all epidermis. Searching for interacting factor is in progress.
