Abstract
Aldrovanda vesiculosa once could be found in many places of the world except North and South America. The plants propagate by forming axillary buds, and produce winter resting buds in fall which sprout in late spring. Flowering is very rare. It is extremely difficult to maintain the plants. Aldrovanda vesiculosa was cultured and propagated in vitro, then grown outdoors, to prevent its extinction and to conduct morphological and physiological research throughout the year.
Apical segments of Aldrovanda vesiculosa were excised and sterilized in 0.5% NaClO3 and cultured in 1/10 MS liquid medium. The plants proliferated well by forming axillary buds which were excised and subcultured once per month. The propagated plants were taken ex-vitro and kept in ca. 300 plastic containers. The plants in the containers outside kept growing and completed their life cycle, though their growth outdoors was often impeded by the influence of proliferating algae.
