Abstract
Information of species of substances passing through the vascular bundle and their daily changes is limited. We developed a method to obtain the apoplast fluid by pressurizing plant in a pressure chamber. The apoplast fluid was obtained by pressurizing with 0 and 1 MPa at every 4h intervals from 7 to 10-day-old seedlings of Pharbitis nil cv. Violet grown under 16h LD photoperiodic condition. Components of sugar were determined by HPLC-PAD and GC. The concentration of sugar and protein at 1 MPa changed photoperiodically. The concentration of apoplast sugar in the light (4.5mg/ml) was 3 times higher than in the dark. In the light, the Glc, Suc and Fru ratio was 80:11:9, while in the dark were 45:33:22. GC analysis of hydrolyzed apoplast showed that Glc (64%) and Rha (33%) were major, and Xyl, Ara, Man and Gal were minor. A major component of symplast i.e. sugar was Glc (95%).
