Host: The Japanese Society of Plant Physiologists
Pages 604
A genomic clone of Wiv-1 gene for wound-inducible cell wall-bound acid invertase was isolated from tomato. The 5' upstream region (4kbp) of the gene contained some regulatory elements for phloem-specific expression. This region was fused to the beta-glucuronidase (GUS) gene, and then the chimeric gene was introduced to tobacco. In leaves of the transgenic tobacco, GUS activity continued to increase for 96 h after wounding. A strong GUS staining was observed in pollens and vascular tissues of stems close to petioles in transgenics grown in a greenhouse. These data suggest that the cell wall-bound enzyme encoded by Wiv-1 involves in the regulation of transport of sucrose in leaves and in the sink activity of pollens. In addition, results of some experiments for the induction of Wiv-1 gene expression by sugars will be reported as well.
