Abstract
Resistance responses of cultured rice cells was specifically induced by a incompatible strain of Acidovorax (Pseudomonas) avenae and several pathogen-associated molecular patterns such as flagellin, hrpY, hrpW were involved in this induction of resistance responses. To investigate the transcript abundance and expression patterns during the resistance responses, cDNA microarray including 3353 cDNAs from libraries of the incompatible strain inoculated and non-inoculated cultured rice cells were constructed. Using the microarray, gene expression patterns during the infection in cultured rice cells were compared between the incompatible strain and the deficient mutants of several molecular patterns containing flagellin, hrpY, hrpW, and hrc. Total RNAs were extracted from cultured rice cells inoculated after 0.5, 1, 2, 3, 4, 5, 6 hours and labeled during reverse transcription, then hybridized to microarray. Among the genes induced by the incompatible strain, the gene expressions of 8% (Δfla), 4% (ΔhrpW), 2% (ΔhrpY) and 23% (Δhrc) were reduced, respectively.
