Abstract
In general, it is thought that organogenesis is composed of three sequential phases; acquisition of developmental competence for regeneration, commitment to differentiate shoot or root, and formation of determined organs. Arabidopsis ESR1, a member of AP2/ERF transcriptional factor, is thought to regulate induction of shoot regeneration after the acquisition of competence for organogenesis since expression of ESR1 is transiently induced during the shoot regeneration process and ESR1 overexpression greatly increases the efficiency of shoot regeneration. To dissect the subsequent chain of events that culminates in shoot regeneration, we are screening the genes under ESR1 regulation. For the purpose, a cDNA library was constructed by subtraction between cDNAs from root cultures in which overexpression of ESR1 was induced and those in which the overexpression was not induced. At present we are examining expression of cDNAs in the library during shoot regeneration.
