Characterization of rice dwarf mutant, GA insensitive dwarf1 (gid1)

Abstract

Rice gid1 mutant shows extremely dwarf phenotype, which is insensitive to GA. GID1 was isolated by a map-based cloning and predicted to encode a serine hydrolase. From yeast two hybrid assay, GID1 was considered to interact directly with SLR1, which is a negative regulator in GA signaling. Here, we will report the functional relationship between GID1 and GID2, which encodes a F box like protein involved in the degradation process of SLR1.
The amount of SLR1 protein in gid1 was much less than that in gid2, while gid1 showed much severer dwarf phenotype than gid2. We also performed the phenotypic analyses of GID1 and GID2 over-expresser in gid2 and gid1 mutant background, respectively. Based on these results, we predicted that GID1 attacks SLR1 to inactivate its repressor activity in a GA dependent manner.
This work was in part supported by PROBRAIN.

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