Abstract
Bundle sheath cell (BSC) chloroplasts are more or less evenly distributed at immature region of an elongating leaf blade in finger millet (Eleusine coracana), an NAD-ME-type C4plant, and the chloroplasts migrate to the vascular bundle and establish a centripetal arrangement after the elongation has stopped. The centripetal arrangement of BSC chloroplasts can be re-established after disturbance by centrifugation. Although it was reported that actin filaments concern in the arrangement, the molecular mechanism remains to be investigated. At the first step, we tried to visualize the actin cytoskeleton in leaf tissue of finger millet. We could visualize actin filaments that located around plasma membrane or connected to chloroplasts in BSC with immunofluoresence staining method. We also visualized actin filaments that encircled BSC chloroplasts in immature leaf blade or mature leaf after centrifugation. These actin filaments may mediate migration and arrangement of chloroplasts in bundle sheath cell.
