Characterization of a Novel Short Hypocotyl Mutant, dwarf in light 1-like 1-D (dwl1-D) That Caused by Overexpression of a New Member of GH3 Gene Family.

Abstract

GH3 gene was originally found as a rapid-auxin inducible gene in Glycine max. In the Arabidopsis genome, there are 20 members of GH3 gene family, and the expression of the several genes is regulated by auxin and/or light. Recently it has reported that several GH3s showed adenylation activities to phytohormons such as IAA, JA and SA.
We isolated a novel short hypocotyl mutant from approximately 50,000 of activation tagging lines, named dwarf in light 1-like 1-D (dwl1-D). dwl1-D is dominant and has short primary root, epinastic leaves and shows dwarf phenotype in adult stage and loss of apical dominance. We cloned DWL1 and found that this gene was a new member of GH3 gene family. DWL1 was induced by applied exogenous auxin. 35S::DWL1 lines represented phenotype of dwl1-D.