Abstract
For the waterlogging-stress in plants, there are few studies in green leaves although many studies on anaerobic condition in roots have been done. The fern Ceratopteris richardii has trends to have broad sterile fronds in high-humid condition and narrow fertile fronds in dry condition. The 17-kDa protein is abundant in the sterile fronds, and no similar feature was found in homology searching.
In present work, we further studied the 17-kDa protein by the western blotting analysis. Two bands corresponding to the precursor and the mature proteins were clearly appeared in broad sterile fronds of Ceratopteris richardii, but not in roots and petioles. The immunochemical signal was not detected in narrow fertile fronds. No band was appeared in the leaves of Arabidopsis thaliana. This suggests that the 17-kDa protein may be a novel protein which is induced in fern sterile fronds by a high-water condition.
