Abstract
In differentiating tracheary elements ( TEs ), microtubules and actin filaments control coordinately the pattern of secondary cell walls. Transient expression of the mouse talin actin-binding domain and the mouse MAP4 microtubule-binding domain fused to the C-terminus of the GFP allowed us to visualize dynamics of cytoskeletons in 3-dimensions during the secondary cell wall pattern formation. However, simultaneous observation of both the filaments was necessary to analyze the coordinative control mechanism in relation to the wall formation. Therefore, we constructed talin and MAP4 fused to C-terminus of CFP and YFP, respectively. When these genes were transformed to Zinnia mesophyll cells, both the filaments were stained clearly in living cells. Moreover, fluorescently labeled lectin allowed us to visualize the early pattern of secondary cell walls. We will report the coordinative control mechanism of cytoskeletons during secondary cell wall pattern formation revealed by multiple fluorescently labeling technique.
