Abstract
Most proteins are believed to function within cellular pathways by interacting with many other proteins as components of complexes. Recent advances in proteomic analysis have opened up possibilities for identification of protein components of large complexes, leading to our understanding of their cellular functions. Here we present proteomic analysis of protein complexes involved in ribosome biogenesis. Pre-ribosomal complexes were isolated by affinity-tagged trans-acting proteins of our interest, which are responsible for human ribosome biogenesis. Identification of protein components of the complexes by nano LC-MS/MS and MALDI-TOF/MS have resulted in assignments of a number of trans-acting proteins to particular steps of ribosome biogenesis. Comprehensive proteomic analysis of the trans-acting proteins will allow us to know their functions within the pathway of human ribosome biogenesis.
