A Distributions of Unknown Proteins Constructing Intracellular Structures

Abstract

There are organelles having complicated structures and highly differentiated functions in a plant cell. Organelles ,such as chloroplast and mitochondria, mainly consist of products that are encoded by the genes in nucleus genome.
A fuge information about these genes were provided from the genome projects, and next effort is needed to understand "where" "when" "how" and "which" gene product is expressd and localized in a cell.
In this study, We developed a system to visualize the gene products. This system distribute GFP gene with transposon, resulting insertional mutations which would express the fusion protein. This trick makes possible to find out the functional wild type proteins in organelles at the heterozygous generation. It also makes possible to observe phenotype of homozygote in the next generation. Here, we'd like to introduce some succeeded examples.