Abstract
Yeast two-hybrid (YTH) screen provides a powerful genetic approach to study protein-protein interactions. In order to apply YTH screening in genome scale analysis in Synechocystis sp. PCC6803, we have implemented the GAL4-based YTH method and developed a high throughput YTH system by introducing the improved vectors, yeast mating-based screening method, and culture system using the MultiScreen plate.
Using this system, we have examined possible protein-protein interactions with two-component signal transducers and Synechocystis genes whose orthologues are conserved in the Arabidopsis genome. As a result of the screening with 689 genes as baits, a total of 1,248 independent two-hybrid interactions were identified.
As an extension of this line, we initiated large-scale interaction analysis of the Synechocystis genes with no functional annotation. The latest status of the YTH screening and the interaction network thus obtained will be presented.
