Abstract
A cDNA microarray analysis revealed that some genes are present in increased amounts after dark adaptation, but show a decrease after subsequent FR irradiation of the root of Arabidopsis thaliana (Ler ). Six of these genes are water-channel genes (AtTIP1;1, AtTIP1;2 AtTIP2;1, AtTIP2;2, AtPIP1;2, AtPIP2;3)1).
To investigate the FR-light response of water-channel gene expression in detail, we analyzed the mRNA levels of AtTIP2;2, one of the FR-responsive water-channel genes, by Northern blot analysis. After 1 hour of FR irradiation, dark-adapted plants showed a significant decrease in AtTIP2;2 expression. These results suggest that there may be alterations of water transport in the root of dark-adapted Arabidopsis thaliana during 1 hour of light irradiation.
Currently, we are performing northern blot analyses for phytochrome-deficient mutants of Arabidopsis thaliana, and monitoring of water-transport in Arabidopsis thaliana by MRI. Some of our preliminary results will be presented.
1) Sato-Nara et al., 2003, Plant Cell Physiol. 44 suppl.:150
