Analysis of Jasmonate Signaling Pathway Using an Ozone Sensitive Arabidopsis Mutant

Abstract

Detailed mechanisms of the damage to plants caused by ozone have not been clarified yet. To dissect these mechanisms, we use an ozone sensitive-jasmonate semi-insensitive Arabidopsis mutant, oji1. In the previous report, we showed that biosynthesis of ethylene which propagates ozone damage was suppressed by jasmonate which is thought to have defensive roles in ozone injury. During 0.2 ppm ozone exposure, jasmonate contents in oji1 were higher than those in Ws-2 at 4 h. On the other hand, contents of salicylic acid, which promotes ozone injury as well as ethylene, and gene expression of AtPR-1 in oji1 were similar to those in Ws-2 until 12 h. From these results and analysis on global gene expression during ozone exposure both in oji1 and in Ws-2 using microarray chip, the interaction of signaling pathway of jasmonate to ethylene and salicylic acid will be disucussed