Abstract
The genes of building up chloroplasts express in leaves of plants by perception of light. To know the mechanism, we selected mutant ces101 (callus expression of RBCS) of Arabidopsis by activation tagging on the criterion of expression of RBCS-3B in calli. Gene for receptor-like kinase was activated therein, and the same phenotype was reproduced by ectopic expression of the gene, leading to the conclusion that the CES101 is the receptor-like kinase. This gene was markedly expressed in leaves but not in roots in the wild-type line. Intracellular localization of CES101 was analyzed with a CES101/mRFP1-fused protein expressed transiently. Fluorescence of CES101-mRFP1 was observed in Golgi vesicles. Transcriptional-profiling analysis was performed by oligo-microarray for calli of ces101 and its parental line. The levels of transcripts for a variety of genes related to photosynthesis were higher in calli of ces101. We would also report the progress of the analysis of ces102 line.
