Abstract
Microarray analysis was performed to identify genes diurnally expressed under continuous dark conditions in leaf blades or leaf sheaths of rice (Oryza sativa L.). Three diurnally expressed genes were selected to examine their functions. Here, we reported analysis of a gene which had a similarity to a transcription factor gene, Dof. Accumulation of the transcript for endogenous Dof-like gene in each organ was different between sense and antisense cDNA-overexpressing transgenic plants. Transcripts for its transgene in each organ also showed the accumulation patterns similar to those of the endogenous gene. Although the Dof-like gene exhibited diurnal oscillations of its transcript with the peak early in the light period in transgenic plants the same as in wild-type plants, it showed slightly longer peak in transgenic plants. The results of expression analysis of known circadian clock-regulated genes in transgenic plants will be also reported.
