Abstract
In order to elucidate the function of cryptochromes (cry) in rice, we had isolated all rice CRY genes (OsCRY1a, OsCRY1b and OsCRY2 ) and have analyzed the their expression patterns at transcription level.
Here, we examined tissue specific localization of each CRY protein by the western analysis. We transformed rice with expression vectors which overexpressed OsCRY1a or OsCRY1b genes and analyze the effect of cryptochromes on the early photogenesis in rice. We have noticed that coleoptile elongation is inhibited by blue-light irradiation. We compared blue-light sensibility on the inhibition of coleoptile elongation between OsCRY1a or OsCRY1b overexpressed transgenic rice and WT. Very low level of blue light irradiation didn't inhibit the coleoptiles elongation in the WT plants, while OsCRY1a or OsCRY1b overexpressed transgenic plants showed the inhibition. These results indicate that the possibility OsCRY1a and OsCRY1b are involved in the inhibition of coleoptile elongation by blue-light irradiation.
