Abstract
The plant cell wall is a dynamic architecture constructed by many kinds of proteins, most of which are encoded by multi-gene families. Although the complete sequencing of Arabidopsis genome has revealed thousands of putative cell-wall related genes, functions of most of these genes are not known. In order to investigate their roles in the cell wall formation, we extracted cell wall proteins from Arabidopsis suspension -cultured cells with 1M KCl solution. We also extracted proteins from the protoplast which had been allowed to regenerate cell wall for 1h and 3h, respectively. Using the 2D-PAGE and MALDI-TOF MS technologies, we separated, mapped and identified these proteins, and found 72 protein spots in the native cell wall, 176 and 213 protein spots in the 1h and 3h regenerated protoplast, respectively. Based on these results, we discuss roles of these proteins in individual step during the cell wall formation.
