Isolation and characterization of genes induced by ABA and sucrose synergistically in the rice callus

Abstract

In our previous experiments, the AGPase large subunit (AGPL), which is a key enzyme in starch biosynthesis, is induced by sugar in rice callus. Sugar signal transduction pathway is connected to ABA signal transduction pathway has been reported. Callus was treated with ABA to clarify the effect of ABA on AGPL gene expression. AGPL gene expression was induced by ABA plus sucrose (suc) synergistically was 5 fold higher than that by either ABA or suc. cDNA-AFLP analysis was used to obtain specifically expressing genes, which is regulated by ABA plus suc. The expression pattern of 80 fragments out of a total about 10,000 fragments was altered by ABA plus suc. For example, Sucrose Synthase 3 and Hexose transporter related to sugar metabolism and Rice Branching Enzyme 4 related to starch biosynthesis were included in these 80 fragments.