Abstract
Our laboratory has initiated a functional genomics program to study the model plant, a crucifer Arabidopsis thaliana at the transcriptome and metabolome levels. We chose a cultured cell line of A. thaliana T87 which were able to control of biological variation for metabolome analysis. Metabolic profiling of A. thaliana T87 cell culture was performed using multidimensional analysis technique based on the sensitivity and selectivity of mass spectrometry (MS). These analytical systems include reverse-phase HPLC coupled to UV photodiode array detection and electrospray ion-trap mass spectrometer, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometer, and gas chromatography (GC) coupled to time-of-flight (TOF) mass spectrometry. Profiling of the phenolic compounds in A. thaliana T87 cell culture using HPLC/MS detected kaempferol-type flavonoid metabolites accumulating in leaf of wild-type A. thaliana. Another metabolite profiling using GC/MS and CE/MS will be presented.
