22K microarray analyses of the BR regulated genes in rice

Abstract

We have already isolated brd1 dwarf mutant of rice and identified the causative gene, OsBR6ox, a BR biosynthesis gene¹⁾. In this research, we tried to find genes up-regulated or down-regulated by BL using brd1 mutant and 22K rice microarray. Gene expression pattern in the brd1 mutant in the presence of BL was compared to that in the absence of BL. As a result, 21 genes were up-regulated and 20 genes were down-regulated 3hr after the addition of BL compared to the control. While 24hr after the addition, 95 genes were up-regulated and 17 genes were down-regulated. About 90% of the expression patterns of the 43 genes analyzed by real-time PCR were coincided with those of microarray. Further, about 50% of the expression patterns of the analyzed genes by real-time PCR were biologically repeated.
¹⁾ Mori et al., Plant Physiol. 130:1152-1161. (2002)