Abstract
It has been reported that the acid rain influenced the growth and metabolism of plants. However, the influence of acid snow on wintering plant has been hardly reported. In this study, the equilibrium-freezing test under acid condition (acid freezing test) was done to study the response of wintering plant to acid freezing stress, which may be caused by acid snow. When leaf segments of cold acclimated winter wheat (Triticum aestivum L. cv. Chihokukomugi) were applied to the acid freezing test, the survival rate after the acid freeze-thaw (pH 2.0) was markedly decreased as compared with that after the freeze-thaw in pure water (pH 5.6), a control experiment. On the other hand, survival rate after supercooling in the acid solution (pH 2.0) was hardly lowered. These results suggested that the acidic condition in a freeze-thaw process of winter wheat promoted the freezing injury.
