Abstract
A cDNA that encoded mangrin, a novel protein which may play an important role for the salt tolerance of a mangrove plant, Bruguiera sexangula, was previously isolated using a functional screening method. Enhanced growth rates were observed in the transformants that expressed mangrin in both Escherichia coli and tobacco-suspension-cultured cells. Interestingly, the amount of mangrin mRNA in the B. sexangula cultured cells increased within 3 h following the addition of over 50 mM NaCl. However, there are no information about the mangrin promoter region. Therefore, mangrin promoter region was amplified by PCR and the sequence was determined. The sequence analysis indicates that there are four putative W-boxes in the promoter region. The promoter region was introduced into tobacco cultured cells (BY2) and tobacco plant, respectively, and the promoter activity was investigated using beta-glucuronidase gene as a reporter gene.
