Abstract
Phospholipase D (PLD) is the enzyme that hydrolyze phosphatidylcholine and generate phosphatidic acid and choline.
Arabidopsis thaliana encodes 12 PLD genes, which are categorized into plant-type PLDs and animal-type PLDs by their domain structures. Plant-type PLDs are known to play roles in the signal transduction of environmental stresses. On the other hand, AtPLDζ1, one of two animal type PLDs in Arabidopsis, has been proposed to play an important role in the root-hair cell morphogenesis. In this study, We analyzed the expressions-pattern of AtPLDζ2, the other animal-type PLD.
A 3-Kbp promoter region of AtPLDζ2 was fused to GUS gene and introduced into Arabidopsis. The transgenic plants showed the GUS activity mainly in floral organs, indicating that AtPLDζ1 and AtPLDζ2 have different expression patterns.
