Abstract
We reported at the former JSPP meeting that mutation in some genes not involved in photosynthesis could influence the chlorophyll fluorescence kinetics. In our previous report, random mutagenesis was adopted to generate mutants. This method had several demerits: low probability in disruption of small ORFs or difficulty in selecting mutants that grow slowly. To overcome these demerits, we are constructing a genome-wide set of mutants using transposon-inserted DNA at known position. Among 23 mutants constracted so far, 4 mutants showed major different fluorescence kinetics and 5 mutants showed minor different kinetics compared with wild type. Further construction of mutants is in progress aiming at functional classification of genes according to the fluorescence kinetics.
