Abstract
The OsRac1, a small GTPase, functions as a regulator of the production of reactive oxygen species in rice defense response. To know the OsRac1 gene expression pattern, we introduced OsRac1promoter::GFP into rice. The OsRac1::GFP expression was observed in vascular tissue of rachillae, lemma, palea, collar, lateral root primordia, tiller bud and mesocotyle. To examine the reactive oxygen production and the occurrence of cell death in these organs, the DAB and the Evans blue staining in roots were performed. A part of the epidermis and the cortex of parent root and the primordia of lateral root were stained by DAB and the basal region of lateral root in parent root was stained by Evans blue. The lateral root development was inhibited by the treatment with DPI, an inhibitor of NADPH oxidase. These results suggest that the reactive oxygen production by OsRac1 would be required for the root development.
