Abstract
It has been demonstrated that bisphenol A (BPA), an endocrine disrupter with estrogenic properties, is metabolized to BPA-ο- β-D-glucopyranoside in the tobacco seedlings (Plant Cell Physiol. 43: 1036-1042, 2002). This reaction may be catalyzed by BPA specific glucosyltransferase (BGT). In this study, we isolated 3 types of cDNAs encoding BGT by RT-PCR method with RNA of tobacco suspension cultured cells (Nicotiana tabacum L. cv. BY-2). Nucleotide sequence of the cDNAs were completely identical to those of NtGT1a , NtGT10a and NtSAGT (DDBJ Ac. No.AB052557, U32643, AF190634), respectively. Extracts of E. coli cells harboring these cDNAs had BGT activity, indicating that they encode BGT. Transcripts for NtGT10a and NtSAGT were high amount in both 4 weeks-old tobacco (Nicotiana tabacum L. cv. Xanthi NC ) leaves and root. But transcript for NtGT1a was only detected in the leaves. Our results indicate that BGT constitutes a multi-gene family.
