Abstract
The elicitor activity against tobacco BY-2 cells was found in an autoclaved and filtrated Alternaria alternata culture medium. The object of this study is isolation and analysis of the elicitor active compounds from the A. alternata culture medium. The culture medium was autoclaved and filtrated to remove the fungus body. A 99% methanol-insoluble fraction of the filtrated solution showed an elicitor activity. The active fraction was separated by an extraction with 75% methanol, removing protein and dialysis. Finally, the elicitor was isolated by the reverse phase HPLC and gel filtration HPLC. The elicitor activity was measured by BY-2 chitinases induction. The isolated elicitor was composed mostly of carbohydrates and showed elicitor activity with the concentration of 10-30 ng/ml. Hydrolysates of the isolated elicitor were dirived the alditol acetates and analyzed by a gas chromatography. As the results, the elicitor active fraction was composed mainly of glucose (about 80%).
