Abstract
Root gravitropism is an inevitable feature for proper plant growth. However, the mechanism to perceive the gravity and to transmit this signal in roots is not clear. According to the hypothesis that root collumella cells percept the gravity, we searched genes that are ectopically induced in root collumella cells from Arabidopsis T-DNA enhancer trap lines. As a result, we isolated the specific expression genes from 7 lines that induced GFP in root collumella cells. Using three sets of nest primers designed from the T-DNA (Gal4-GFP) sequence, we obtained 45 highly specific amplifications after Tail-PCR. Sequence determination showed that 2 lines had the T-DNA region inserted near the genes for a Myb family transcription factor and an auxin responsive protein, respectively.
We are now searching the knockout lines and identifying the promoter regions for these genes.
