Functional Analysis of Arabidopsis AtREV1 and AtREV7 Genes which are Involved in the Error-Prone TLS

Abstract

The error-prone DNA translesion synthesis (error-prone TLS) has been well characterized in yeast and mammalians, but not in higher plants. Recent finding of an AtREV3-disrupted mutant (rev3-1) in Arabidopsis suggested that the error-prone TLS is significant for tolerance to DNA damages in higher plant. To clarify the details of the error-prone TLS in higher plants, we analyzed the T-DNA inserted Arabidopsis mutants defective in AtREV1 or AtREV7 genes, which are thought to be involved in the error-prone TLS system. The AtREV1 mutant (rev1-1) was sensitive to UV-B, gamma-ray and MMC. These physiological responses of rev1-1 were quite similar to those of rev3-1. The results indicated that AtREV1 functions in the error-prone TLS in Arabidopsis. We will also present a couple of results which characterize the T-DNA inserted AtREV7 mutant.