Abstract
Stable isotope labeling methods possess methodological advantages because of its secure and diverse properties, whereas radio isotope labeling methods widely used in biological studies have disadvantages in terms of these aspects. Nuclear Magnetic Resonance (NMR) is a powerful method for identification of labeled metabolic flux and their structures. We focused major metabolic nucleus in plants, carbon and nitrogen, and performed their multi-dimensional NMR analysis. Briefly, we harvested 15N labeled seeds of Arabidopsis thaliana, and observed specific nitrogen movements upon water absorption or light irradiation in vivo. Furthermore, 13C labeled samples exhibit distinct metabolic difference between wild type and a mutant strain of Arabidopsis thaliana. A validity of NMR in plant metabolomics will be discussed.
