Abstract
In this study, ascorbate peroxidase (APX), L-galactono-1,4-lactone dehydrogenase (GLDH), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GSH) in broccoli florets were investigated in the gene expression after harvest, treatments with 10% sucrose and irradiation of various light intensity. The decreases in ascorbate and sucrose contents in florets were caused at 48 h after removing leaves from intact plants with simultaneous suppression of the gene expressions of chloroplastic APX, MDAR, DHAR and mitochondrial GLDH, but not when the plants were kept intact or leaves attached. In addition, ascorbate contents were kept high and the gene expressions related to chloroplastic and mitchondrial ascorbate metabolism were enhanced by feeding 10% sucrose or high light in harvested broccoli florets.
