Abstract
Effects of methylviologen on global gene expression of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 were studied by DNA microarray. In our previous report, we found that the Fur-homolog, Slr1738, plays a regulatory role in induction of sll1621 in response to oxidative stress. Here, detailed analysis of the DNA microarray allowed us to identify sll1620 and slr0589 as possible target in addition to sll1621. By gel mobility shift assay, we demonstrated that the His-tagged Slr1738 specifically bound to the upstream regions of sll1620 and slr0589. Furthermore, we found another set of oxidative stress-responsive regulon: transcriptional regulator Sll0088 and slr0074-slr0075. This was confirmed by specific binding of the His-tagged Sll0088 to the upstream region of slr0074 in gel mobility shift assay.
