Abstract
MADS-box gene was postulated to be related to anthocyanin biosynthesis. As a MADS-box gene regulated proanthocyanidin biosynthesis in Arabidopsis. We tried to isolate MADS-box gene from red root of sweet potato. Degenerate primer was designed based on amino acid sequence of MADS domain of MADS-box genes, then 3'-RACE was performed. A new MADS-box gene was isolated, which was named IbMADS1. The expression of IbMADS1 was analyzed by northern blot analysis. The maximum transcript level was observed in floral buds, pink roots beginning color and red roots fully colored. A lower expression was found in developing tubers. The transcript was not detectable in flowers at anthesis, buds, leaves, stems and white roots. The expression of anthocyanin biosynthetic gene, CHS, CHI, F3H, DFR, ANS and UFGT, were also analyzed. We will report the possibility that IbMADS1 is related to anthocyanin biosynthesis base on our results.
