Abstract
It has been known that the genes for the enzymes involved in anthocyanin synthetic pathway express in specifically differentiated tissues and organs. The PAL gene (gDcPAL3) plays the most important role in the induction of anthocyanin synthesis in carrot suspension cultured cells regulated by 2,4-D. Deletion analysis of the gDcPAL3 promoter indicated that the promoter region between -258 and -194 were required to give full activity. This region contained the GCC sequence, which has been known as the cis-element of the ERF family having the plant specific DNA binding domain, AP2/ERF domain. Previously, we isolated two AP2/ERF transcription factors, DcERF1, DcERF2, that interact with the GCC sequence of the gDcPAL3 promoter. However, the function of these genes was unknown. Transactivation functions of DcERF1, DcERF2 using carrot suspension cultured cells will be reported.
