Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 45
Conference information

Purification of the Inhibitor of Rubisco from Sink-limited Sweet potato Leaves-2
*Nobuyuki IboriMito IwafuneNaoki HashimotoMinobu KasaiShinich Sawada
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Pages 534

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Abstract
Decrease in photosynthetic activity of sweet potato source-sink model plants placed under sink-limited conditions was mainly due to the binding of inhibitor to Rubisco. This inhibitor was suggested to be a sugar phosphate by analysis using HPLC.
The phenols contained largely in leaf extract from sweet potato disturbed purification of inhibitor using activated Rubisco. Moreover, pectins contained largely in the extract adsorbed strongly the inhibitor. Therefore, purification of the inhibitor and analysis of the inhibitor by NMR was prevented.
In order to obtain the inhibitor in high purity, it is under examination about the removal method of an impure ingredient.
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© 2004 by The Japanese Society of Plant Physiologists
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