Abstract
To identify genes expressed in the procambium and/or vasculature, we have been creating enhancer-trap lines in Arabidopsis and screened for the lines expressing a reporter gene in the vasculature. The enhancer-trap T-DNA includes GFP as a reporter gene and pBluescript II for the plasmid-rescue method to isolate genomic DNA adjacent to the inserted T-DNA. So far, we have screened about 20,000 T1 transformants and obtained 346 lines in which GFP fluorescence was detected in the procambium and/or vasculature of rosette leaves (105 lines), roots (209, out of which 10 are specific to the root apex), shoots and roots (16), and shoots except rosette leaves (16). Besides further screening T1 transformants, we are currently isolating genomic DNA adjacent to the T-DNA insertion sites by TAIL-PCR and plasmid-rescue procedures to identify genes responsible for the vasculature-specific expression.
