Abstract
To dissect vascular pattern formation, we have isolated Arabidopsis van3 mutant showing the fragmented minor veins. For the aim to unveil the molecular mechanisms of the VAN3, we performed yeast two hybrid screening. ADL1A, a GTPase dynamin, was isolated as a VAN3 interacting protein. From the analysis of ADL1A::ADL1A-GFP transgenic plants, ADL1A can be co-localized with the endosome marker FM4-64. ADL1A expression was observed in the provascular cells of the torpedo-stage embryos, trichomes, and stigmatic papillae. adl1a mutants produced unbranched trichomes, abnormal isotropically expanded papillae cells, and discontinued veins. Furthermore, adl1a adl1e double mutant showed embryo lethality without producing the procambium cells. These results suggested that the ADL1A would widely regulate the single cell differentiation step, and in some cases ADL1A activity could be regulated by the VAN3 GAP.
