Abstract
7SL RNA is an RNA component of Signal Recognition Particle (SRP). It is thought that 7SL RNA was derived from ancient tRNA, therefore transcribed by RNA polymerase III. In plant 7SL RNA genes have USE (Upstream Element) and TATA sequences as transcription regulatory elements at upstream of transcribed region, and don't have internal promoters like as tRNA or 5S rRNA genes. In mammal 7SL RNA have propeller secondary structure at 5' region of RNA, and contributing auto-regulation of transcription. In this study, we introduced point mutation into Arabidopsis 7SL RNA genes and mutated genes were used as templetes for in vitro transcription assay with tobacco nuclear extract, From this assay, it is strongly suggested that a cis-regulatory element located in transcribed region and RNA was destrabilized when a mutation introduced as dissolving propeller motif which was also located in 5' region of Arabidopsis 7SL RNA.
