Abstract
Plant morphogenesis is controlled by a complex system with many genes.
MADS-box genes are well known as regulating organogenesis of flowering organs, and also morphogenesis of vegetative organs, leaves and shoot. Morphogenesis of roots development also may be controlled by this kind of genes. We are trying to isolate MADS-box genes regulating root development of radish (Raphanus sativus L.). mRNAs were extracted from the early stages of root development, and subjected to RT-PCR and then to 3'RACE with degenerate primers designed from amino acid sequence of MADS-domain. We have successfully isolated MADS-box genes having MADS-domain and K-domain, characteristic pf plant MADS-box genes. They are homologous to AGL12, AGL15, AGL20, AGL24, SVP, FLC, FUL/AGL8, respectively. Their expression patterns were analyzed during root development of radish by Northern blot.
